播种机组纯作业时间利用率与地块条件的匹配模型与试验

机械化播种作业是现代农业生产的重要环节,纯作业时间利用率是衡量播种机组作业效率的重要指标。该研究依据农业机组运行机理,在调查研究的基础上,提出季节时间利用率概念,确定了播种机组的作业时间构成,建立了播种机组纯作业时间、转弯时间、加种肥时间的计算模型和3种加肥方式下的播种机组纯作业时间利用率计算模型,明确各参数随地块面积、地块长度等条件变化的一般规律;以此为基础,在机组正常作业状态下,采用定距离多点多设备同步跟踪测的试验方法获取4种典型播种机组各类作业时间数据,并运用3σ原理剔除无效数据;依据所建模型及有效试验数据,采用Matlab 2012b对4种播种机组的纯作业时间利用率随地块条件变化规律进行模拟仿真,并采用Sigmaplot 12.5软件直观表达试验结果;进而探索了播种机组纯作业时间利用率随地块条件化的原因,确定了目标时间利用率条件下不同播种机组与其所适宜的作业地块面积和地块长度的定量关系:播种机组纯作业时间利用率大于0.6时,约翰迪尔7830机组适合作业地块面积大于等于6 hm^2、地块长度为1 200~1 400 m;维美德171机组适宜作业地块面积大于等于6 hm^2、地块长度为1 000~1 200 m;常发504机组适合作业的地块面积大于等于1 hm^2、地块长度为500~1 500 m;黄海254机组适合作业的地块面积大于等于0.3 hm^2、地块长度200~1 500 m。研究成果可为不同地块条件的播种机组选型提供定量依据。     

Quantitative aspects of solid-state C-NMR spectra of humic substances from soils of volcanic systems

Cross-Polarisation Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance spectroscopy (CPMAS ¹³C-NMR) represents one of the most powerful tools to investigate soil organic matter (SOM) mainly because of its inherent capacity to provide a semi-quantitative evaluation of carbon distribution. A critical parameter during acquisition of CPMAS ¹³C-NMR spectra is the contact time required to obtain the cross-polarisation between proton and carbon nuclei. The procedure to evaluate the best contact time for the acquisition of a quantitative CPMAS ¹³C-NMR spectrum is to perform Variable Contact Time (VCT) experiments. In this work the structural features of a number of purified humic substances from Italian and Costarican volcanic soils were investigated by CPMAS ¹³C-NMR spectroscopy after having performed preliminary VCT experiments. The VCT experiments showed that the average contact times vary according to the origin and chemical structure of the humic material. The optimal contact times (OCT) for nine humic samples were between 250 and 800 μs These values were different from the time of 1000 μs that is commonly applied as the best average contact time for humic materials. Moreover, by comparing the NMR data to those obtained by elemental analysis (C/H ratio), it appeared that the efficiency of the cross-polarisation between protons and carbons, and hence the contact time, is affected not only by the number of protons, but also by their distribution over the molecules. The evaluation of errors in quantitative estimation of the different carbons revealed that the use of OCT generally reduced by half the loss of signals occurring when the average contact time of 1000 μs is used in CPMAS ¹³C-NMR spectra of humic substances.     

沪池沟流域水平梯田建设研究

沪池沟流域水平梯田建设研究孙天星，郑合英（黄河水利学校水土保持专业科，开封市４７５００１）沪池沟流域位于河南省西部洛宁县境内，系黄河二级支流，属黄土丘陵沟壑区第三副区，流域面积２３．２９ｋｍ２。该流域１９８８年被列为黄河中游水上保持综合治理试点小流域...     

山西省草地资源人口承载量研究

为了在黄河中、上游地区加快实施中央“退耕还林还草恢复生态环境”的决策,正确估算区域人口的承载量是一项基础性的研究工作。以山西省和山西省黄河流域为研究区域,利用相关统计、试验数据,设计不同草地利用率层次和不同肉食消费水平等次,对山西省和山西省黄河流域及其各县市草地资源人口承载量进行了单项和组合型的动态变化研究,为山西省区域退耕还林还草和生态移民工作提供了理论基础。     

厦门市2001-2002年PM10浓度时间序列变化分析

在厦门市环境大气污染物中，PM10是其首要的大气污染物。厦门市大气中的日PM10浓度受天气和气候以及土地利用类型、地表植被覆盖度等诸多因素的共同影响而呈现出一定的周期性，应用时间序列方法对厦门市2001～2002年的PM10浓度进行周期性和趋势性的分析，揭示了厦门市大气环境中PM10浓随时间变化的周期性规律。同时讨论了部分气候因素对PM10浓度年内变化的影响。     

丘陵红壤橘园不同地面管理措施的经济生态效益研究

在丘陵红壤橘园上．经过3年6种地面管理措施的经济生态效益研究。结果表明：（1）水土流失，以清耕法作对照，生草法、克无踪、草甘膦、草草克、克克草的地表径流量可显著减少分别为38．8％．42．5％．18，7％，28．7％和37．5％；土壤侵蚀量可显著减少分别为55．5‰，51．7％．39．9％．46．8％和50．0％；流失的氮磷钾养分总量可分别减少60．3％．50．2％，37．0％．41．8％和45．4％。（2）杂草再生率，以生草法做对照，则处理中的清耕法、克无踪、草甘膦、草草克和克克草分别是生草法的55．1%．67．2％．30．3％．36．8％和51．2％。（3）土壤肥力，以土壤OM，N,P2O3,K2O总量增减率看，清耕法为-4．4%,生草法7．1％，克无踪6．9％，草甘膦5．3％．克克草6．2％．草草克6．6％。（4）柑橘的产量和品质，以清耕法作对照，除生草法外，克无踪、草甘膦、草草克、克克草均增产7％～10％；可溶性固形物、总糖量、总酸度、糖酸比以及单果重均达到显著差异水平。克无踪调控杂草效果显著．可作为丘陵红壤丘陵园地水保与培肥兼得的有效地面管理措施。     

Bioassay-directed Fractionation of Organic Extracts of Marine Surface Sediments from the North and Baltic Sea - Part II: Results of the biotest battery and development of a biotest index (8 pp)

-

Part I: Determination and identification of organic pollutants Part II: Results of the biotest battery and development of a biotest index

-

Preamble. This series of two papers presents the results of an interdisciplinary research project (ISIS) dealing with bioassay-directed fractionation of marine sediment extracts. Part I presents the extraction and fractionation procedure as well as the results of chemical analysis, including non-target analysis of sediments. Part II describes the results of the biotest battery in relation to chemicals possibly causing parts of the observed effects. A biotest index is used to compare the toxicities of the samples.

-

AUTHORS / AFFILIATIONS Ninja Reineke (3), Werner Wosniok (4), Dirk Danischewski (1), Heinrich Hühnerfuss (3), Angelika Kinder (5), Arne Sierts-Herrmann (5), Norbert Theobald (2), Hans-Heinrich Vahl (6), Michael Vobach (1), Johannes Westendorf (6) and Hans Steinhart (5).

-

(1) Federal Research Centre for Fisheries, Institute for Fishery Ecology, Palmaille 9, 22767 Hamburg, Germany (2) Federal Maritime and Hydrographic Agency, Bernhard-Nochtstr. 78, 20359 Hamburg, Germany (3) University of Hamburg, Institute for Organic Chemistry, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany (4) University of Bremen, Institute of Statistics, Bibliothekstr. 1, 28334 Bremen, Germany (5) University of Hamburg, Institute for Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany (6) University of Hamburg, University Hospital Hamburg-Eppendorf, Department for Toxicology, Vogt-Kölln-Str. 30, 22527 Hamburg, Germany (7) Eurofins Wiertz-Eggert-Jörissen, Stenzelring 14b, 21107 Hamburg, Germany

Goal, Scope and Background

The ecological relevance of contaminants in mixtures is difficult to assess, because of possible interactions and due to lacking toxicity data for many substances present in environmental samples. Marine sediment extracts, which contain a mixture of environmental contaminants in low concentrations, were the object of this study. The extracts were investigated with a set of different biotests in order to identify the compound or the substance class responsible for the toxicity. For this goal, a combination of biotests, biotest-directed fractionation and chemical analysis has been applied. Further on, a strategy for the development of a biotest index to describe the toxicity of the fractions without a prior ranking of the test results is proposed. This article (Part II) focuses on the biological results of the approach.

Methods

The toxicological potential of organic extracts of sediments from the North Sea and the Baltic Sea was analyzed in a bioassay-directed fractionation procedure with a set of biotests: luciferase reporter gene assays on hormone receptor and Ah receptor, arabinose resistance test, fish embryo test (Danio rerio), comet assay, acetylcholinesterase inhibition test, heat-shock protein 70 induction, oxidative stress and luminescence inhibition test (Vibrio fischeri). The test results provided the basis for the calculation of a biotest index by factor analysis to compare the toxicity of the samples and fractions.

Results and Discussion

Results of 11 biotests on different fractionation levels of the samples were described and discussed with regard to the occurrence of contaminants and their toxic potentials. Polychlorinated biphenyls, polycyclic aromatic hydrocarbons, quinones, brominated indoles and brominated phenols were in the focus of interest. A biotest index was constructed to compare the toxic responses in the samples and to group the biotest results.

Conclusion

The procedure presented in this study is well suited for bioassay-directed fractionation of marine sediment extracts. However, in relatively low contaminated samples, high enrichment factors and sufficient fractionation is necessary to allow identification of low concentrations of contaminants which is required to link effects and possible causes. In the present case, the relation between substances and effects was difficult to uncover due to relatively low concentrations of pollutants compared to the biogenic matrix and to the remaining complexity of the fractions. The results, with respect to the brominated phenols and indoles in the samples, highlight the successful use of bioassay directed fractionation in the case of high concentrations and high toxicity.

Recommendation and Outlook

In general, it has been shown that a marine risk assessment requires focusing on the input of diffuse sources and taking into account the fact of mixture toxicity. Effects resulting from biogenic substances will make the assessment of the influence of anthropogenic substances even more difficult.     

干粉PAM溶解时间对土壤饱和导水率的动态影响

本试验选取两种质地土壤(黏壤土和砂壤土),采用3种干粉PAM施用水平(0、22.5 kg/hm~2和45 kg/hm~2),测定土样在10.25 mm/h入渗速度下的土壤饱和导水率(KS),然后根据土样团聚体含量和稳定性及团聚结构的微观图片,分析干粉PAM影响下土壤结构的变化特征,进而说明干粉PAM溶解时间对KS的影响机理。结果表明:施用PAM后,KS随干粉PAM在水中溶解时间的延长而逐渐减小,最终趋于稳定;干粉PAM溶解时间较短时,PAM处理的KS高于对照,其中PAM施用水平45 kg/hm~2时砂壤土KS提高幅度最大,较对照提高26.87%,但不同PAM施用量处理间的KS差异不显著。干粉PAM溶解时间足够长时,PAM处理的KS均显著低于对照,其中PAM施用水平45 kg/hm~2时黏壤土KS降低幅度最大,较对照降低10.86%,但是不同施用量处理间KS差异不显著。从影响机理上分析,PAM主要是通过增加土壤团聚体含量及稳定性来提高KS;而干粉PAM溶解时间足够长时,由于PAM易吸附土壤颗粒,水解后的PAM分子链不断伸张延长,堵塞了土壤孔隙,加上PAM本身的黏滞特性,从而降低了KS。研究干粉PAM溶解时间对KS的动态影响,可以为PAM在改善土壤导水能力方面的应用提供理论依据。     

协助自然修复微量元素污染酸性土壤研究：8年田间试验

There are many remediation techniques for organic contaminated soils,but relatively few of them are applicable to trace elementcontaminated soils.A field experiment was carried out to investigate assisted natural remediation（ANR） of an acid soil contaminated by As,Cd,Cu,Zn and Pb using one inorganic amendment,sugar beet lime（SL）,and two organic amendments,biosolid compost（BC）and leonardeite（LE）.The experiment was arranged in a completely randomized block design with four treatments in three replicates:1) a non-amended control（NA）;2) SL amended at 30 Mg ha^-1 year^-1;3) BC amended at 30 Mg ha^-1 year^-1 and 4) LE amended at 20 Mg ha^-1 year^-1 plus SL amended at 10 Mg ha^-1 year^-1（LESL）.The amended plots received two doses of each amendment（DO2）:one in October 2002 and another in October 2003.The plots were then divided in half into two subpolts and one subplot received another two doses of the same amendments（DO4） in October 2005 and October 2006.In 2011,the pH values of the amended soils were greater than that of the NA soil,with the SL-amended soil showing the highest pH.Total organic carbon（TOC） was also greater in the amended soil,and greater with DO4 than with DO2.Amendments reduced the concentrations of 0.01 mol L^-1 CaCl₂-extractable Cd,Cu and Zn,especially in the SL-amended soil.Plant cover of colonizing vegetation was enhanced by amendments,but was independent of amendment doses.Changes in soil properties from 2003 to 2011 showed that the first amendment application of DO2 caused a high differentiation between all the amendment treatments and the NA treatment.After the second application of DO2,soil pH and TOC continued increasing slowly.Further two applications of amendments（DO4） caused differences only in the organic treatments.Plant cover increased over time in all the treatments including NA.It could be concluded that the slow and steady natural remediation of this soil could be enhanced by amendment application（ANR）.     

风干土保存时间和湿土培育时间对黄淮海平原潮土酶活性的影响

以经历18年不同施肥管理的土壤为研究对象,阐明它们经过4个不同时间保存或处理后的土壤脲酶、转化酶、脱氢酶、及FDA酶活性的动态变化。处理包括:风干保存30天或鲜土状态、风干保存210天、风干土湿润至田间持水量(25℃)条件下分别培育15天和51天;同时评估这些酶活性的变化程度与土壤本身有机碳含量之间的关系。结果表明,风干土保存时间和风干土湿润后短期培育均对脲酶活性影响很小,但风干土湿润培养51天后其活性则显著降低;随风干土保存时间延长,转化酶活性显著降低;与鲜土相比,风干土湿润培养15天后,脱氢酶活性显著提高,但继续湿润培养至51天后,其活性又降至与鲜土相当,因此风干土湿润培育一定时间后测定的脱氢酶活性可用来代表其田间自然湿度时的状态;FDA酶活性的变异程度最大,与其从鲜土状态至风干状态的活性急剧下降有关。土壤本身有机碳含量与脲酶和脱氢酶的活性变化程度成显著负相关关系,说明土壤有机碳含量是决定它们随环境条件改变而变化的主要因素之一。另外,土壤NH4+-N、NO3--N和可溶性有机碳含量对上述4种处理的响应程度也存在差异。其中风干状态土壤经湿润培育处理后,NH4+-N含量呈先降后升趋势,正好与脲酶活性变化趋势相反;而NO3--N含量整体上呈上升趋势,可溶性有机碳含量则正好相反。